human specific gfap  (TaKaRa)

Journal: Stem Cells Translational Medicine

Article Title: DLK1-expressing neural progenitor cells promote tissue repair and functional recovery after cervical spinal cord injury

doi: 10.1093/stcltm/szaf014

Figure Lengend Snippet: DLK1 expression modulates the in vivo differentiation of transplanted NPCs. (A) Representative immunohistochemical images of injured spinal cord sections transplanted with control NPCs or DLK1-NPC, stained for hNSE (human neuron-specific enolase, a neuronal marker), STEM123 (human GFAP, an astrocyte marker), and hGSTπ (GSTπ, an oligodendrocyte marker). Positive cells appear violet/purple as a result of the Vector VIP HRP substrate. Scale bar: 40 μm. (B) Violin plots showing the average number of hNSE⁺, STEM123⁺, and hGSTπ⁺ cells (cells/mm²) in the injured spinal cord from both NPC and DLK1-NPC groups. The central white line indicates the median, and the upper and lower white lines represent the first and third quartiles, respectively. Statistical analysis was performed using unpaired two-tailed t-tests (* P < .05).

Article Snippet: Additionally, the following human-specific primary antibodies were used: human neuron-specific enolase (hNSE), a marker for neurons (1:500; R&D Systems, MAP5169), human-specific GFAP (STEM123), a marker for astrocytes (1:500, Takara Bio.

Techniques: Expressing, In Vivo, Immunohistochemical staining, Control, Staining, Marker, Plasmid Preparation, Two Tailed Test

Journal: PLoS ONE

Article Title: Human Glial-Restricted Progenitor Transplantation into Cervical Spinal Cord of the SOD1 G93A Mouse Model of ALS

doi: 10.1371/journal.pone.0025968

Figure Lengend Snippet: Antibody Details.

Article Snippet: Astrocytes were identified using pan-GFAP (1∶400; polyclonal; Dako; Glostrup, Denmark) or human-specific GFAP (1∶5,000; monoclonal; Dako) .

Techniques:

Journal: PLoS ONE

Article Title: Human Glial-Restricted Progenitor Transplantation into Cervical Spinal Cord of the SOD1 G93A Mouse Model of ALS

doi: 10.1371/journal.pone.0025968

Figure Lengend Snippet: Transplanted HuNA + hGRPs differentiated into GFAP + astrocytes by disease endstage in gray matter both near the injection site ( A ) and at distances up to 6 mm ( B : image represents region with migrating cells approximately 3 mm caudal to the injection site), as well as in white matter ( C ). At disease endstage, approximately 50–80% of all HuNA + cells co-expressed the astrocyte marker GFAP at sites within 1 mm rostral or caudal of the injection sites, regardless of whether cells were found in gray ( A ) or white ( C ) matter ( D ). This percentage decreased at greater distances from the injection sites ( B , D ). In addition to the pan-GFAP marker, transplanted-derived astrocytes were labeled with the human-specific GFAP marker ( E – F ). Only a minor fraction (approximately 1%) of HuNA + cells co-expressed the microglial marker, Iba1 ( G – H ). Arrowheads denote HuNA/GFAP double-labeled cells in panels A and C . Scale bars: 50 µm.

Article Snippet: Astrocytes were identified using pan-GFAP (1∶400; polyclonal; Dako; Glostrup, Denmark) or human-specific GFAP (1∶5,000; monoclonal; Dako) .

Techniques: Injection, Marker, Derivative Assay, Labeling